Coding

Part:BBa_K4765003:Design

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-08-08)


Nb1, 3AKH13 from 10.1016/j.cell.2018.06.041


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 40
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence of T7-RBS should be inserted before the CDSs.


Source

bacteriophage T7 gene 10

References